I am trying to define % reproducibility of a specific bioreactor. Basically I have three identical reactors that are used as triplicates for the same experiment and identical testing. So I would like to calculate in a simple way how similar are the resulting values in an attempt to define reproducibility of results of the reactor. What I did was to calculate how different the resulting values were and subtract this from 1 (the 100% similarity) with this formula (1- VR/AVE)*100 where VR is the value range (xhigh – ylow) and AVE the mean average. I also calculated the % coefficient of variation with StDev/Ave*100 where StDev represents standard deviation. However, I'm not sure if this is sufficient or correct? Anyone with some ideas?
Dear Urania Michaelidou
In order to ascertain reproducibility of Bioreactor runs, first establish the operating ranges of the variables such as pH , temperature, aeration capacity etc,. Assuming that you have successful runs having the parameters with in their operating ranges, the main product concentration and quality can be studied for reproducibility between runs.
You may require to have six runs to apply statistical calculations. Figure out on the acceptability criteria to call them reproducible. You can apply 1SD, 2SD and 3SD , for required confidence interval of 68%, 95% and 99.3% similarity.
Best Wishes,
Krishna Mohan
I consider the coefficient of variation and intervals of confidence as enough to verify reproducibility ... as Krishnamohan Bk said you can apply 1SD, 2SD and 3SD to generate the intervals of confidence, that is:
inferior limit = AVE - (3*SD)
superior limit = AVE + (3*SD)
Thank you for taking the time to answer Krishnamohan Bodduluru Javier Ulises Hernández Beltrán , Paulette Cully
. Very helpful! Thanks a lot. I will see how I can implement.- Badges
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