03 July 2021 0 8K Report

I am looking to construct some spin columns using microcentrifuge tubes and was hoping for some best practices and pro tips. The amount of glass wool to use, bed volume and sample volume are the primary concerns.

More Gibson Arias's questions See All
Are you are carrying out research to solve algal bloom management problems?

Please consider submitting a manuscript to our special issue "Scientific Advances for Understanding, Predicting, and Managing Harmful Algal Blooms" in the Journal of Environmental Management...

07 June 2024 7,841 1 View

What happens to natural extracts (mainly antioxidants) in very alkaline environment?

Hello everyone, Im concerned about the bioactive properties of different natural powder extracts (botanicals and mainly rich in antioxidants) in a very alkaline environment, specifically during...

29 January 2024 1,516 5 View

En Sistemas de Generación Distribuida para austoabastecimiento de usuarios, es necesario colocar un medidor adicional al Medidor Bidireccional?

In Distributed Generation Systems for self-supply of users, is it necessary to place an additional meter to the Bidirectional Meter? Certain benefits of the additional meter in consumption: - To...

07 January 2024 6,746 3 View

Could anyone help me to identify this Ampelisca sp.?

It was found in a rocky intertidal from Southern Spain. One of the most notable characters is a merus prolonged anteriorly in a peg-shape in the pereiopod 7, covering most of the carpus. Its...

13 November 2023 9,822 1 View

Help with GFP and ChAT co-staining?

I am trying to co-stain for GFP and a ChAT marker in the brain of a mouse model that has GFP-expressing neurons. Typically I get a strong fluorescent signal when I stain only for GFP (photo 1),...

18 September 2023 5,866 2 View

Has anyone worked with comercial gingival epithelial cells?

Hello, I am going to start working with gingival epithelial cells. However, I have not found any studies concerning the only commercial line I have found (HGEpiC). Is there anyone who has worked...

18 September 2023 1,396 0 View

How accurate is biofluid FTIR spectroscopy in diagnosing patients with cholestatic liver disease?

Fourier Transform Infrared Spectroscopy (FTIR) has been widely used by scientific researchers in the field of cancer diagnosis and treatment, using various kinds of biofluids and tissues. The...

05 September 2023 8,516 1 View

What happen to vegetable polyphenols in a lipid environment?

Why natural polyphenols (like proanthocyanidins from berries) decrease their antioxidant activity in a lipid environment? vs an aqueous environment? I know that existing research suggests that...

12 July 2023 4,490 2 View

How are the physical characteristics of a loudspeaker and its enclosure related to a mathematical circuit model of the loudspeaker?

I am trying to find a mathematical model of a loudspeaker in an enclosure. By this, I mean an equation that describes the electrical impedance of the speaker as a function of the physical...

23 June 2023 3,191 1 View

What type of graphene or carbon nanotube is best for concrete?

I am researching several methods of adding graphene and carbon nanotubes to concrete in order to increase the strength. Many papers have said that they added graphene, but no real descriptors of...

13 June 2023 265 6 View

Similar questions and discussions
Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

How can women be responsive when they can make love for hours?

… with my present female lover … she and I spend anywhere from two hours to six hours in caressing, touching, cuddling, hugging, lip kissing, deep kissing and intimate conversation before,...

11 August 2024 4,521 0 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Hi, please what is the best tool on Aspen plus that can help me get the best inlet temperature and pressure for cryogenic distillation?

I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...

06 August 2024 4,611 0 View

How to perform EEG source analysis on each trial of data separately?

Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...

30 July 2024 2,744 1 View

Best Practices for Constructing and Maintaining Gravel Roads in Rural Areas?

Hello, I'm involved in a project focused on constructing and maintaining gravel roads in rural areas. We're particularly interested in ensuring durability and reducing maintenance costs over time....

29 July 2024 9,623 0 View

What are the best practices for washing and preparing fruits and vegetables to reduce pesticide residues?

What are the best practices for washing and preparing fruits and vegetables to reduce pesticide residues?

27 July 2024 9,147 4 View

Can anyone kindly suggest research topics in the area of thermodynamics, heat transfer, fluid mechanics or renewable energy?

I am an undergraduate mechanical engineering student seeking suggestions on topics related to either thermodynamics, fluid mechanics, heat transfer or renewable energy for my final year project....

24 July 2024 4,058 5 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close