Can you recommend medium recipe for growing aob and nob bacteria in same medium?

hello, I want to grow aob and nob bacteria in the same medium is that possible? if it is can you give me a medium recipe? (what I mean by the same medium is that I want to grow them in the same...

29 December 2020 3,115 1 View

As an undergraduate, I need a bit of assistance on finding/coming up with a research topic to study together with a researcher at my institution?

I am undergraduate, majoring in public finance, also enthusiastic about data science and analysis. I would like to do research with a researcher at my institution who is studying in the areas of...

12 October 2020 8,227 6 View

How do We Maintain Appropriate Cell microenvironment with 3D Cell Culture Method?

3D Cell Culture is newly optimized and promising discovery in cell organization research and other researches that needs evindences in cell culture (in vitro) level. Are 3D Cell Cultures mimic...

23 September 2020 7,109 4 View

How do We Clarify the Effects of Drugs on DNA Replication?

DNA Replication and its components are common targets in discovering new drugs as antimicrobial therapeutic agents especially while dealing with antibiotic-resistant species and other diseases...

21 September 2020 6,172 4 View

How can I solve this error in Abaqus topology optimization?

I was trying to perform a topology optimization process in Abaqus. However, I faced an error which I attached the screenshot of. How can I disable the offending rule mentioned in this error message?

21 September 2020 6,732 1 View

How the smart grid would shape sustainability policies in a large democracy ?

The role of smart grid shaping a large democratic system ??

10 August 2018 2,512 3 View

TA cloning problem. Is it possible two or more inserts to ligate with a plasmid in TA clonig ?

I did TA cloning with instaclone PCR cloning kit and I got some weird results. I picked 12 white colonies, isolated them and cut with a single cutter enzyme BamHI (I cut with BamHI to linearize...

18 July 2018 6,305 5 View

What is the total angular momentum of the universe?

In some books I read that the total angular momentum of the universe is close to zero but I could not find a reference. Can you suggest one?

30 January 2018 2,229 2 View

How to find in ANSYS ALPD and BETD value?

Hello everyone, I am using ansys but I am somewhere I have calculated ALPAD and BETAD values but I want to enter ALPD and BETD values in its material. how to find these values in ANSYS.Thanks in...

21 January 2018 8,584 1 View

What can be the reason of this kind of SDS-PAGE image?

I have a solution including 4% mechanically deboned chicken meat protein. Solution pH is approximately 12. I am using 12% seperating and 5% stacking gels. Sample buffer consists of 25 mL TRIS-HCl...

05 May 2016 3,096 12 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can I perform two way ANOVA in case of some missing groups?

I had control cells and performer ELISA analyses group (time 0). I stimulated cells with substance A and B and cultivated for 1, 2 and 3 hours. On each cells I performed ELISA. I have results...

17 February 2021 8,933 4 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

What is the relationship that determines the depth of the mechanical cultivator with the age or size of the weed For crops planted in lines?

the great depth of the cultivator may lead to passage under the roots without killing them and may give negative results to break up the soil below the roots, increasing their activity, especially...

03 February 2021 3,198 7 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View