When I analyzed oligonucleotides by LC-ESI-TOFMS with HFIP-TEA system, the charge-state distribution was different in two batches of experiment but same method. How to explain this and how to obtain higher charge-state on LC-ESI-TOFMS using current mobile phase system.
Thanks
I suggest you check extremely carefully all your experimental parameters, especially on the MS. Even small changes can have dramatic effects on the appearance of the spectra. This may well be your case.
Maybe your mobile phase composition has altered between experiments. In case you use a nano-LC, part of the mobile phase can evaporate directly through the plastic tubing connecting the bottles with your pump. Therefore either prime your LC pump modules or change the mobile phase completely using freshly prepared solvents.
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