I have 3 solution samples need to normalized.
sample 1: UV spectra showed absorption solution at 450 nm was 0.254 (abs. units) and PL intensity peak at A value
sample 2: UV spectra showed absorption solution at 450 nm was 0.260 (abs. units) and PL intensity peak at B value
sample 3: UV spectra showed absorption solution at 450 nm was 0.244 (abs. units) and PL intensity peak at C value
--> HOW can I normalized the PL data for the case I want to compare PL or three samples?
Do you want the value of emission intensity keeping the absorbance same?
If so, divide the emission spectra by {1-10^(-Absorbance)}. Use the absorbance at the excitation wavelength. This is same as calculation of quantum yield.
To make a fair comparison excitation wavelength should be same for all samples. Also to minimize inner-filter effect the absorbance should be low (0.05) at the excitation wavelength.
I agree with most of the statements above but would go one step further.
If you want to compare the PL intensities why not simply measure the Quantum yields of each of the compounds and then compare the quantum yield values?
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