We have some TEM images of some Pd nanoparicles. We can see the lattice of these Pd nanopaticles. Does anyone know how to calculate the lattice spacing of these Pd nanoparticles? It should be around 0.22nm. I have tried ImageJ using some methods that I found by googling. However, all of them seemed not right.
There is one
https://imagej.nih.gov/ij/docs/examples/tem/
But this method does not work for my ImageJ. I don't know why.
The answer here provided by
https://www.researchgate.net/post/How_to_I_know_the_lattice_planes_d-spacing_from_a_HRTEM_image_by_using_Image_J_software
I followed this method but the d is not around 0.22nm.
Tao Ye, the simple method of using a profile plot gave me an answer of 0.19nm, which is close to what you are expecting. This was just on one set of 13 planes. I would recommend that you repeat it wherever you can see the lattice planes and average your results. After setting the scale (it is a little difficult to know exactly where to measure as the ends of the scale are thick and not at a definite points), draw a line perpendicular to the lattice planes within a domain and generate a profile. Count the planes and do the division. I have added an image of what I did on your sample.
Tao Ye, the simple method of using a profile plot gave me an answer of 0.19nm, which is close to what you are expecting. This was just on one set of 13 planes. I would recommend that you repeat it wherever you can see the lattice planes and average your results. After setting the scale (it is a little difficult to know exactly where to measure as the ends of the scale are thick and not at a definite points), draw a line perpendicular to the lattice planes within a domain and generate a profile. Count the planes and do the division. I have added an image of what I did on your sample.
You can use the Digital Micrograph software from gatan, and manually calibrate the image if you are yousing non-gatan camera. Offline DM software is free-to-download from gatan homesite.
I measured 0.23 nm. Better calibrated image should give you more reliable results, though.
PS I totally agree with Ian. +1 for his reply!
You can also calculate the lattice spacing by fast fourier transformation.
Be careful when you have overlapping lattices (Moire fringes). Your analysis could be erroneous.
Ravi, can you provide Toa Ye with a worked example of the FFT method using his data?
@ Tao
You can follow the steps given in this site and perform FFT
https://imagej.nih.gov/ij/docs/examples/tem/
I have downloaded the image given in the site and performed FFT analysis on it. Please find the original image and the FFT of the image as attachments.
If you send me a representative file, I can do it for you as Ian mentioned.
The preview of FFT of tem file does not seem to be available. It is a bmp file, just click on the hyperlink to download the file.
How can we manually calibrate the image in GATAN if we are using the non-gatan camera
Hi Tao Ye ,
Here is a tutorial video link: https://youtu.be/zkMTfbRpDnI
I hope, you will get solution in this small video.
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