I am currently proposing a study on the metal uptake of a particular plant. In the study, I am going to spike my pot experiments in treatments with varying concentration of the metal of interest. One of the parameters I want to find is BCF. I am aware that it is a basically a ratio between the concentration of metal in roots/concentration of metal in the soil. I am confused however with this concentration of soil. Am I supposed to use the concentration of soil after the remediation or the concentration of the soil before the process in solving for the BCF? Thank you and God Bless!