After picking out key broad themes under a given topic of inquiry, is it okay to build a proposed conceptual framework before the data collection starts (which is reflected in my research questions and interview guide) and then build on specific themes under those broad themes as the data collection unfolds? Or would such an approach create bias in the study as I have already identified key themes and would ask the respondents questions in and around those themes? For example- In a study trying to understand the key factors at the individual, societal, and institutional level which contribute to unmet needs/ barriers to care for HIV patients in India, is it okay to categorize themes into- individual, societal, and institutional levels and ask questions on those? Or should I go for data collection with no such structure in mind?