I'm looking for a protocol to apply simulated herbivory treatment on plants by spraying methyl jasmonate or jasmonic acid. If anyone has a detailed lab protocol to share that would be much appreciated!
I am also wondering, what is the difference between using MeJa or JA for the treatment? Is there any difference in terms of the expected response from the plant?
MJ does not simulate herbivory, because for that you need to establish it as a direct proxy, which is hard. But it will simulate plant defence in general. Use MJ, which is the actual regulator, and spray when stomata are open, since the cuticle wax may repel the chemical :)
Hello Kristiina; You might find some useful information in this paper.
Thaler, J. Jasmonate-inducible plant defences cause increased parasitism of herbivores. Nature 399, 686–688 (1999). https://doi.org/10.1038/21420
Best regards, Jim Des Lauriers
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