I am working on stimuli-responsive controlled cargo delivery in living cell using surface functionalized mesoporous silica nano particle with Plasmid/ DNA as a pore closing gates. I have synthesized the functionalized MSNPs, and I have met a problem in further step, that is MSNPs are being aggregating while addition of plasmid/DNA to MSNPs for complexation of MSNPs with Plasmid/DNA.
Can anyone suggest how to avoid the aggregation of MSNPs during complexation with DNA and why it is happening.?
Technical information:
I used +Ve charged functionalization to make a complex with -ve charged Plasmid.
using 10:1 w/w, MSNP:plasmid (1mg: 0.1mg in 1mL) solutions.
thanks for ur suggestions.