How to attach adult mouse hepatocytes to glass slides for immunofluorescence?

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Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

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IHC on mouse liver hepatocytes?

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Glass fiber content in a gypsum board ?

Any idea on how to get the volumic ratio of glass fiber in a gypsum board ? Thank you for your help !

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Concrete compressive strength databases?

I am looking for datasets similar to this one: http://archive.ics.uci.edu/ml/datasets/Concrete+Compressive+Strength I am looking for concretes with different types of aggregates (Recycled,...

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How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

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Recalcitrant seed short-mid storage method?

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How to properly calculate the fluid solid heat transfer between polypropylene matrix and glass fiber in a porous medium?

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IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

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How to properly calculate Interfacial area density in a porous domain?which are possible value for glass fiber?

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Todd Jensen

Many articles for use with hepatocytes in culture list Collagen I as a coating protein. Whatever you use I recommend characterization before and after to be sure the coating hasn't modified the phenotype.

http://www.ncbi.nlm.nih.gov/pubmed/23577314

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4290156/

Michael Delannoy

Have you tried charging your microslides with Poly-L-lysine?

Hasan Al Faruque

Poly-L-lysine or collagen coated cover glasses may help to attach the cells.

Yuri P Rubtsov

Thanks a lot for helpful hints; as usual I have to try both to figure out the best option.

Hasiena Ali

Hi Yuri, We culture our cells on glass coverslips, allow them to attach with medium in small petri-dishes. Then go on to stain them and finally mount them with a slide before viewing.