How to assess pathogens and non pathogens in fresh larvae?For this is there any biochemical test or kits are available..?

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Debra Sharon Ferdinand-James

I had to check in RG for related studies to respond to your question as it's not my area of expertise. I hope the attached are helpful for you.

Best regards,

Debra

https://www.researchgate.net/search.Search.html?type=publication&query=pathogens%20and%20non%20pathogens%20in%20fresh%20larvae&previous=publication

Adam B Shapiro

I would feed or inject the larvae with the suspected pathogen at various doses and see if they (the larvae) get sick (stop eating, stop moving, lose weight, turn color, ...) and/or die. This would be classified as a bioassay.

Houda Kawas

Dear Abdul aziz Soomro

First of all, Identification the causative if its Microorganism or Chemical, by Koch's Postulates: to Identify the Causative Agent of an Infectious Disease, and bioassays ,there are kits for different causal agents, also you could detect it by microscope , isolate it on different medium. even the larvae alive you could get some blood by syringe or culture there Stools caterpillar droppings, you may found some bacterial exudates .

Pls. find the attached files, may help in your research. Good luck

T. W. Fisher, Thomas S. Bellows, L. E. Caltagirone, D. L. Dahlsten, Carl B. Huffaker, G. Gordh. 1999. Handbook of Biological Control: Principles and Applications of Biological Control Academic Press,Pp. 1046. ISBN0080533019, 9780080533018

https://www.researchgate.net/profile/Margaret_Skinner/publications

http://www.life.umd.edu/classroom/bsci424/BSCI223WebSiteFiles/KochsPostulates.htm

http://www.invasive.org/hwa/hwa.pdf

Regards

Prof. Houda Kawas

Abdul aziz Soomro

I am very thankful to you for showing your keen interest @ scientist Debra Sharon Ferdinand, Adam B Shapiro, Houda Kawas.. I got much more ideas..

Hans Rediers

Dear Abdul aziz Soomro,

We tested virulence of Vibrio anguillarum strains in sea bass larvae. The protocol is described in the paper below. It is a robust assay to assess the virulence.

regards,