How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Can I arbitrarily take a cycle number of 40 for the gene that was not expressed at all in qPCR results calculation?

I ran RT-qPCR for a control and a treated group and it appears that my gene of interest was not expressed at all in any of the subjects in the control group. So I was wondering if I could...

20 February 2021 6,243 10 View

Why the protein moved out of the solvation box after minimization using OpenMM?

Hello, I am trying to perform MD simulation for the protein-ligand system and I got this after Minimization. I used openMM for minimization and I used ambertool for protein-ligand...

20 January 2021 1,922 2 View

Where can I buy BrHPP (bromohydrin pyrophosphate) for cell cultures????

I need to get BrHPP for expanding Tgd cells, so if anyone knows about a biotec/chemistry company that has it commercially available I would appreciate the information. Thanks!

20 December 2020 7,022 3 View

In Ansys HFSS, how do I replace all instances of a Local Variable within a Design with another variable?

I have created a fairly complex antenna that has a parameterized wire radius (wire_r), however I would like to change the name of the variable to better reflect its purpose (e.g. wire_r_cu). Since...

04 November 2020 7,168 1 View

Is Uniform Convergence property contradictory to No Free Lunch Theorem?

In Statistical Learning Theory, Uniform convergence means for a hypothesis class H, given m(\delta, \epsilon), for arbitrary distribution over domain (X, Y), with probability $1- \delta$, the...

03 November 2020 7,732 5 View

If one wants to link together power sources all across North America, would it be better to use DC transmission lines, or gas pipelines, say with H2?

Intermittent renewable energy sources become more efficient and reliable as the number and diversity of sources is increased. But electrical transmission over distances greater than 2000 miles...

20 October 2020 731 5 View

HX temp1 out as a function of flow rate1 in?

Trying to derive a transfer function for a cross flow tube and fin HX (liquid to air, tube is air side & hot side). Temperature control feedback loop, temp sensor for feedback and valve for...

23 August 2020 1,738 3 View

What is the meaning of Positive delta G In Free Energy calculation between protein and Ligand?

I have done Gromacs for FEP ( Free energy perturbation) between Protein and Ligand in Water solvent, with Positive Ions. I got DG 26.02 +/- 3.14 Kj/Mol. I got positive Delta G. How i can...

18 August 2020 736 4 View

Time in While Loop ?

Hello , I have a problem . I want to calculate the time in m / s from While loop so that I can integrate the values. I tried a lot, unfortunately that didn't work. I use the MPU6050 accelerometer...

03 August 2020 4,518 3 View

Are there alternatives to Interval Type 2 fuzzy logic control?

We are working with complex systems with intermittent renewable energy sources, and AC grid dynamics, hence Fuzzy Type 1 does not adequately deal with the uncertainties. Providentially, the...

14 July 2020 5,650 4 View