What are the solvent requirement to study protein conformational changes via NMR?

I wanted to study the conformational changes taking place in gamma D crystallin (eye lens protein) at low pH via NMR. What would be the preferred solvent requirements for this particular protein...

31 December 2020 2,103 4 View

How to add randomness to a channel using Matlab simulations?

I have to simulate multipath fading channels and add randomness to it.

21 September 2018 6,377 2 View

What is the best method for analysing anthocyanins in plants ?

Anthocyanins in plant extracts

13 September 2015 1,366 4 View

Wat is the precursor for depositing Calcium using vacuum deposition for Ca Test of barrier permeation?

I need to prepare the setup for Ca test for WVTR measurement. I am not sure how to deposit calcium on my glass substrate . We don't have ALD.

02 October 2014 3,057 2 View

How long can one store bisulfite converted DNA and at what temp so that it does not affect the quality of pyrosequencing?

I used 4 month old stored DNA @ -20 deg C. I got good PCR bands but not good result for pyrosequencing. Would it help if I store @ - 80 deg C and make aliquots of that?

04 May 2014 8,606 3 View

What is the mechanism behind the fluorescence in ester compounds?

Does the ester group act as donor or acceptor?

26 January 2014 635 4 View

What kind of innovative services should be provided to promote the accessibility of products and services of public library by underprivileged person?

01 January 1970 4,548 3 View

Could you please recommend a cytoplasmic counterstain for immunocytochemistry?

I am using ICC to stain some nuclear proteins in muscle-derived stem cells. I'm currently using a HRP-conjugated secondary and DAB substrate (From Biogenex) I would like to avoid haematoxylin as a...

01 January 1970 4,694 3 View

Factor analysis in case of small sample size and no. of variables more than sample size

I am working on a small dataset with 22 observations and using 30 variables under eight dimensions to prepare an index. I am using factor analysis for the same. But the condition for factor...

01 January 1970 7,418 3 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View