I am currently undertaking the synthesis of AgNP utilizing plant extract as a bioreductor. The synthesis procedure involved reacting the plant extract with 1 mM AgNO3 while optimizing the ratio. Various ratios of AgNO3 to plant extract were explored, including 5:5, 6:4, 7:3, 8:2, and 9:1. Subsequently, UV-Vis characterization was performed to identify the ratio that yields a single peak in the range of 380-420 nm with the highest absorbance. Upon determining the optimal ratio, the synthesis was scaled up to a total volume of 250 mL. However, post-centrifugation at room temperature, only a minimal pellet was obtained, with the colloidal solution predominantly adhering to the bottom of a small 15 mL tube.