The normal (baseline) level of glutamate in the frontal cortex of rodents varies depending on several factors, including:

• Species (rat vs. mouse)
• Strain
• Sample preparation (whole tissue homogenate vs. microdialysate)
• Detection method and sensitivity of the ELISA kit

However, in general:

• Tissue homogenates: Glutamate levels are often reported in the range of 5–15 µmol/g tissue (or 5000–15000 nmol/g), depending on brain region and extraction method.
• Microdialysis samples: Extracellular glutamate concentrations in the frontal cortex are typically much lower, around 1–5 µM under basal conditions.

For example:

• Tilleux et al. (2007) reported basal extracellular glutamate levels around 1.5 µM in rat frontal cortex using in vivo microdialysis.
• Tissue studies using ELISA or HPLC often show higher total glutamate concentrations in the micromolar to millimolar range.

So yes, there is no universal standard, and values can vary based on experimental design. It’s best to compare with control values reported in studies using similar methods and brain regions.

Let me know if you'd like references or ELISA-specific papers

Many thanks for your answer and I would be very thankful if I found a paper that could guide me to the control level of glutamate in male wistar rat frontal cortex by ELISA

You're most welcome — I'm really glad my previous answer was helpful.

Regarding your question, I did a deeper search, but it seems most studies measuring glutamate in the frontal cortex of male Wistar rats rely on HPLC, enzymatic colorimetric assays, or microdialysis, rather than ELISA specifically.

That said, for tissue homogenates, glutamate levels are generally in the range of 5–15 µmol/g tissue, while extracellular levels (via microdialysis) are typically around 1–2 µM.

If you're applying ELISA to whole tissue samples, this range should offer a reasonable reference. I also came across a few protocols that might be similar to your work:

• BioVision Glutamate Assay Kit (colorimetric/fluorometric, applied to tissue homogenates)
• Abcam Glutamate ELISA Kit (ab83389), which has been used in brain tissue samples from rodents
• MyBioSource Glutamate ELISA Kits, sometimes applied in preclinical neurotoxicity models

If you’re using a specific kit or targeting extracellular glutamate, I’d be happy to help you find more targeted studies or protocols. Could you please share a bit more about your experimental setup? (e.g., tissue vs. dialysate, sample prep method, ELISA kit brand)

Looking forward to your reply