EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Why is my qPCR suddently not working?

I've been working with qPCR for some months now. I amplify 2 genes (PSEN2 and HN1), and the protocols are optimized for both and have been working fine. The other day I amplified HN1 for some...

21 February 2021 3,437 3 View

Can ethanol be used as an analgesic?

If ethanol has analgesic properties, why isn't it used for clinical purposes?

18 February 2021 8,227 4 View

Acid phenol:chloroform (25:24:1) for RNA extraction instead of (125:24:1)?

Hi everyone! I am about to extract RNA for RNA sequencing assays and my Invitrogen kit/protocol...

14 February 2021 1,755 3 View

Which journals (Q1,Q2) accept simulation based design and results of antenna if fabrication and testing facility is not available?

I need a list of good (preferably Q1,Q2) journals that will accept research papers prepared on designs done with the help of simulation software e.g CST, HFSS etc.

08 February 2021 9,636 5 View

Method to measure printed layer thickness on paper substrate ?

I want to measure thickness of screen printed graphene ink on paper substrate. Can anyone suggest any accurate and feasible method to measure it. I tried profilometry (stylus & optical) but...

11 January 2021 6,179 6 View

Are there any public datasets of consumer complaints about service industries at the European level?

06 January 2021 1,718 2 View

No title

02 January 2021 7,172 4 View

Does anyone know how to get a scopus or sciencedirect report of the trends in recent years on a specific research topic?

I am trying to obtain a trend report on a specific issue at your instigation in recent years

28 December 2020 848 2 View

No title

16 December 2020 9,657 5 View

ML Techniques to find an optimal value?

I have a small program that I can configure via a simple input number (integer) called a 'grain size'. I've noticed that the run time can vary based on this number's value. I wrote up a genetic...

20 March 2020 5,063 3 View

If anybody could suggest me any professors of screenplay writing?

Film Industry in Pakistan, as a matter of fact, could not at all be on its feet----There are numerous reason contributing to the dilapidated state of the affairs of Film, but I hold a fragile...

03 April 2017 4,363 0 View

If any one could share with me research articles and/or theses on screenwriting?

I intend to do my PhD on screenplay writing. Research articles or theses on various aspects of screenplay please be shared.

08 September 2016 2,446 0 View

Is anyone interested to network with researchers in Screenwriting, Screenwriters, Filmmakers, Experimental Filmmakers & Screenwriters?

contact Alison Metoudi [email protected]

31 December 2014 6,615 2 View

What was the binder used in the project?

I am Manu Ravuri working in the area of 3D printing. I am in search of a binder for printing of copper powder. Can you suggest a binder for the powder.

01 January 1970 506 1 View

*Recent Trends in Management: Opportunities and Challenges* Volume 1

*Recent Trends in Management: Opportunities and Challenges* Volume 1 DOI 10.17605/OSF.IO/DHFJ7 *Call for papers:* The Department of Management, St. Claret College, Bengaluru, Karnataka,...

01 January 1970 7,824 3 View