I used a relatively low dose of STZ (45 - 55 mg/Kg) and hyperglycemia appeared within 3 days (to be sure the rats will maintain hyperglycemia after a week), but I have noticed some tips I would share them with you as I think they would be helpful.
- STZ causes initial hypoglycemia within hours, so it would be better to give the rats diluted glucose solution after 2 - 4 hours of the injection. This gave me less death cases and did not interfere with diabetes induction.
- Rat sensitivity to STZ and the degree of hyperglycemia differ according to age, STZ dose and the administered food, and as I noticed, high fat diet makes rats highly susceptible to diabetes and needs small doses of STZ to develop it.
- The rats that do not develop diabetes (hyperglycemia) within a week tend to be STZ resistant and if you inject them again with STZ, they would not respond.
Best regards,
If done correctly, all rats treated with STZ should be diabetic in less than 24hr.
Hyperglycemia will occur in 24 h after STZ injection. Then, you must wait longer to see diabetes complications in different organs
Intraperitoneal injection of 70 mg/kg Streptozotocin can cause Hyperglycemia (up to 300mg/dl) and glycosuria (++++, equivalent to 111 mmol/L) after 24 hours in the Sprague-Dawley rats.
Ref. Polychromatic LED Therapy in Burn Healing of Non-Diabetic and Diabetic Rats, Journal of Clinical Laser Medicine & Surgery, Vol. 21, Number 5, 2003, Pp249-258.
If is just to cause hyperglycemia, a small time it's ok. However, for sustainable changes on diabetes complications (nephropahty,...), longer duration is needed, it depends on the aims of the study!
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