How to deal with the recudtion of amplification efficiency of indexed primers?

Hi all, I am now working with ITS2 sequencing library preparation. I had observed that after introducing adapters, linkers and barcodes to a primer, the indexed primers have a much lower...

27 January 2021 5,045 3 View

Are there any DNA intercalators that do not have fluorescence?

I understand several normal DNA intercalators, such as ethidium bromide, acridine, SYBR et al. would emit fluorescence. Is there any kind of DNA intercalator that do not have fluorescence?

25 December 2020 688 3 View

How to interpret the centrality indices in network analysis?

I learned that in order to interpret the centrality indices, the CS coefficients resulted from a bootnet test are preferably above 0.5 and above 0.25 is acceptable. In my network, the CS...

29 October 2020 3,545 3 View

How to create a graph of psychopathology symptoms when conducting network analysis in R?

I've been working on the network analysis of adolescent depressive symptoms. I've estimated the network, the centrality indices and network stability using packages qgraph and bootnet. Then I want...

22 October 2020 5,591 3 View

How to interpret the results of NetworkComparison Test when conducting a network analysis in R?

25 September 2020 9,936 2 View

Does anybody know how to use the Lagranto model?

These days I wanted to run the Lagranto model to track water vapor transport path. But I got some trouble in installing an available version of Lagranto model. I attempted to use "conda install -c...

04 July 2020 5,417 4 View

Abaqus & Fortran complier linking problem ?

Hi, I am trying to run a vumat subroutine, but there is some abaqus-fortran linking issue. I use abaqus 6146 Micrasoft visual studio 14.0 parallel studio XE 2016 I added these command in my .bat...

25 February 2020 5,698 2 View

Health informatics: Relationships between industry development and academic research?

In digital health domain, is the academic research that leads the development of the industry OR is the industry development that impacts the associated academic research ?

10 June 2019 5,214 6 View

Fluorescence Polarization Assays?

When I titrated fluorescence probe by Protein A alone or by Protein B alone, it do not should any binding signals, mP do not change. However, when I titrated the same fluorescence probe by...

18 March 2019 2,468 3 View

How does esiRNA from Sigma compare to Dharmacon's SMARTpool siRNA?

I would like to know information about esiRNA form Sigma for the following points: 1. transfection protocol; 2. knockdown efficiency; 3. off target effects; 4. toxicity. Anyone would like to share...

31 May 2018 9,661 2 View

How to locate and collect the pineal gland in the amphibian brain?

Our team has been trying to locate and collect the pineal gland in the American bullfrog's brain (Lithobates catesbeianus) for some time now. However, we don't have a standardized protocol for...

18 February 2021 3,453 4 View

Plated Secretory Tissue from Udder--What type of cells are these?

Hello all! I plated some secretory tissue from the udder of a dairy cow. I am trying to isolate mammary epithelial cells. I found these cells on one of my plates, are these developing mammary...

28 October 2020 7,768 3 View

Preparation of mouse thymus for Flow Cytometry?

Hi, I'm trying to develop a protocol for prepping thymus tissues in mice. We will be using NSG mice in our lab and the goal is to get good consistent single cell suspensions to stain for FACS....

26 October 2020 10,006 3 View

How Selenometheonine helps on the growth of bMECs (bovine mammary epithelial cells) under stress conditions?

I'm searching for articles related to "How Selenometheonine helps on the growth of bMECs (bovine mammary epithelial cells) under stress conditions?". Anyone can help?

08 October 2020 8,431 3 View

Tissue Digestion with proteinase k gives "invisible" globule"?

I'm digesting a few different mammalian tissues with a spin kit and proteinase k. Some tissues (esp. testis and pituitary gland) look fully digested and clear. But once the lysis buffer is added,...

09 August 2020 4,443 2 View

Do you have any idea about the clinical significance of diffuse low density of thyroid gland ?

i noticed that the thyroid gland of some patients is quite low, similar to that of muscles. and the CT scan was described for various reasons, even including regular health checks. i tried pubmed,...

29 June 2020 3,238 6 View

Is there any way to clean this type of discoloration of stainless steel?

We are mainly using our vessel made of SS 316L to produce herbal extracts (thyme, marshmallow,...). After each production we are CIP cleaning it with either acidic of basic detergents and final...

07 May 2020 5,198 2 View

Thymus tissue not staining after whole body perfusion, does it need any treatment other than fixation?

I am trying to stain the mouse thymus tissue with no success after doing a whole body perfusion (4% PFA) on euthanized mice, followed by fixation with 4%PFA overnight, followed by sucrose gradient...

02 March 2020 2,800 2 View

Could someone Labelled CD8 T cells in deparaffinized section?

Hi I am trying to labelled murine CD8 LT by immunofluorescence on thymus and tumor sections embedded in paraffin and dewaxed. I meet some difficulties. I tried several Antobodies without...

08 December 2019 4,451 1 View

What is the best fixative for mice adrenal glands either 4% PFA or 10% NBF?

Anyone working on adrenal gland histology, could you please please the protocol to prepare paraffin-embedded mouse adrenals sections? Thank you

09 October 2019 4,674 4 View