Since gentamicin absorbs ultraviolet and visible light poorly and the molecule lacks fluorophores, no direct spectrophotometric or fluorometric method can be applied.
I did the fluorometric method for determination of gentamicin (use of ninhydrin and OPA reagent) but I couldn't get the best calibration curve. When I use ninhydrin for the quantification of gentamicin I didn't see any color change (in 1-20 μg.mL-1) and when I use OPA reagent I couldn't measure the fluorescence of the standard samples (cut off).
We bought the gentamicin sulfate salt from the validate factory.
Please look at the following below links which may help you in your analysis:
https://www.japsonline.com/admin/php/uploads/1741_pdf.pdf
https://pubmed.ncbi.nlm.nih.gov/10708399/
https://www.imedpub.com/articles/quantification-of-gentamicin-by-microbial-assay-technique-and-reverse-phasehplc.pdf
https://www.beckmancoulter.com/wsrportal/techdocs?docname=/cis/4T052/%25%25/EN_GENTAMICIN.pdf
http://hera.ugr.es/doi/16518342.pdf
Thanks
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