I have few unidentified pure algal cultures, by microscopic observation the morphology it look like Scenedesmus sp., Chlorella sp. and Monorophidium sp. Now I want to cross check those culture by molecular identification.
Thus please tell me how I should I construct primer for there identification?
Shunmugavel Chinnadurai
Dear Priyanka,
Central Marine Fisheries Research Institute, Kochi doing molecular taxonomy of marine algae for long time. They will give better answer for you.
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Neha Arora
You can go for 18s rRNA sequencing using universal primers ITS1 and ITS4. After sequencing you can use MEGA6 for assessing the phylogeny. Hope this helps.
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