How I can measured excitation wavelength of alizarin red S as example?

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Yuri Kalambet

You have to start measuring emission of the solution containing molecule at some emission WL and vary excitation WL. The whole curve (emission intensity for every excitation WL) is excitation spectrum, and the lowest excitation WL for which you get response different from zero is the lowest excitation WL. If you know chemical structure of the substance, you can guess excitation WL for some group (it is known from textbooks).