How GSH and 2ME are given in ROS scavenging for cell culture?

More Sheng-Chieh Hsu's questions See All

What is the meaning of borderline passivity?

hey, when i read some papers and books about corrosions of metal materials, there are some descriptions like "The 400-series stainless steels will pit in propionic acid solutions, probably because...

15 April 2024 3,003 1 View

Does anyone know where I can find sector scan ultrasound image data?

I wonder if anybody knows where I could find and download some sector scan ultrasound images? I need them for doing my homework.

07 April 2024 1,764 1 View

Why the published article is not in the author confirmation list?

I recently used ResearchGate's Conform Authorship for the first time. When I was checking the box, I found that the article published by Ian Westbry and I was not in the list. I would like to ask...

14 February 2024 7,769 3 View

Are there any new techniques to study gully erosion at this time? Is machine learning a good approach?

Should we go ahead and build a certain model specifically for the study of gully erosion? Can machine learning be applied to it?

27 December 2023 9,948 3 View

What are the current methods that can be applied to the study of agricultural resources and environmental technology?

For example, how to apply machine science Xi study SOC, can this be counted as an environmental problem?

22 December 2023 6,027 2 View

How to avoid background (perhaps non-specific band?) in Northern blotting?

Hi, I've been working on DIG-labeling northern blotting for a while, but recently i get some troubles regard to the background. As the picture below, i keep having these non-specific bands in my...

04 November 2023 4,672 0 View

Why do we get E0-0 energy value by the crossing point of normalized absorption spectra and emission spectra using 1240/λ ?

why do we get E0-0 energy value by the crossing point of normalized absorption spectra and emission spectra using 1240/λ ?

02 October 2023 6,971 1 View

How should I study multi-agents system properly?

In my opinion, multi-agents system will be a big trending as soon as possible. Multi-agents system can grant to solve real complex problems. But to build a multi-agent system which has many...

13 September 2023 5,367 0 View

Which plasmid extraction kit did you used to prepare plasmids for nucleofection used in human embryonic cell lines?

Hi, I am wondering if different plasmid extraction kits yields different quality of extracted plasmids, which subsequently affected some downstream analysis such as nucleofection in human...

28 August 2023 7,411 3 View

What is the most effective way to insert a 20kb large insert into human genome?

Hello. I intend to insert a 20kb gene cassette into human genome using the CRISPR-Cas9 approach coupled with homology arm directed repair. It is know that this approach is less efficient due to...

13 August 2023 8,381 1 View

GSH estimation assay: What is the right choice of standard?

Hi there, My question is: What standard curves should be used while estimating Tot GSH and GSSG by kinetic method using GR enzyme mediated recyling with DTNB chromophore? Actually I am following...

01 August 2024 8,217 1 View

Could GSH level be more in CCL4 rats than negative control rats?

i compare between the antioxidant and oxidative stress level between negative control rats (saline) and CCL4 intoxicated rats. i found the level of GSH and TAC is more in toxic group than normal....

26 July 2024 1,887 0 View

How could I determine antioxidant activity of Capsicum annuum fruits?

I need a detailed protocol of any suitable method for ex., DPPH to determine antioxidant activity.

28 June 2024 3,855 2 View

Can I dissolve DPPH in THF for antioxidant activity Assay ?

My compounds are soluble In THF, so I woould like to know If for antioxidant activity I can prepare DPPH in THF. Before, I did a test in wich I had DPPH in ethanol, but when I Add my sample in...

17 June 2024 4,183 3 View

Depletion experiments fo these three antioxidants in Atlantic Salmon?

Are there depletion experiments for these three antioxidants in Atlantic Salmon?

16 June 2024 1,915 1 View

Why does the antioxidant activity increase as the number of OH groups on the phenolic ring increases?

and Why do double bonds reduce it ?

13 June 2024 4,836 4 View

Please what should I do if I find a disorder when testing antioxidant activity by DPPH?

I have solubilized DPPH in methanol and used ascorbic acid as a positive control, and am testing the activity of pectins.

05 June 2024 6,197 1 View

How to store fish tissue sample for proximate analysis and antioxidant enzyme analysis?

I need to analyse proximate composition in whole fish and antioxidant enzyme analysis. What is the protocol to store these samples for maximum efficiency in the results. I have -80 degree C...

05 June 2024 1,372 6 View

How does the use of rosemary (Rosmarinus officinalis) as an antioxidant affect the efficacy and safety when taken with iron supplements?

Currently, there is a significant issue of iron deficiency among many people, leading to widespread use of iron supplements. However, many are unaware that iron can be toxic, and excessive iron...

24 May 2024 6,865 1 View

Collaboration on medicinal plants?

Would anyone be interested in collaborating on research focused on the antioxidant and antibacterial activity of medicinal plants?

23 May 2024 6,760 4 View

Luay A. Al-Helaly

GSH and 2ME are not stable enough, but with good cold it will stay longer.

Sheng-Chieh Hsu

Muntaha Abdul-Zahra Ati

HGSH has a role in converting GSH to GST that works to detoxify and is considered another alternative to GSH

Lassaad Hedhili

Intersting question

Chunfeng Xu

2ME is100uM.