Can anyone kindly share on how to prepare chloroform (alcohol free)? the chloroform (alcohol free) will be used for chloroform fumigation-incubation method to estimate microbial biomass C and N . thank you in advance.
Since Alcohol (not sure which one you are talking, MeOH or EtOH) is water soluble and Chloroform is not, you can do 2 - 3 washes with distilled water followed by molecualr sieve 5A or 4A drying step. The water washes should take the alcohol out of the chloroform phase.