Hello all,

I got a PointGray USB3 camera and a F210B firewire cameras. I’m interested in following changes in time of different fluorescence and reflected light in a diverse set of samples. In this type of cameras the timestamp (label of the time point where a frame has been captured) is stored in the first 4 pixels of an image if ones uses 8 bit depth or in the first 2 bytes if the image is 16 bits.

The timestamp is in cycle counts and stored as bit counts in the pixels. The company has a script example to get the info written in C. But I do not know how to code in C nor I have the time to sit down and learn it (unfortunately). I need to convert this bit counts in either cycle counts or seconds. Note that even if you set up the camera to take 1000 images per second, the output is < 1000 and never constant. Questions:

(1) Has anybody tried to get the timestamp of such images in Image J? If so, how do you do it? Note that what I need is to transform the bit count into cycles. I know how to extract the raw info from the sequence.

(2) If not, does anybody has a suggestion?

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