How do you determine the menaquinone of bacteria from the pictures obtained from Jasco MD-2015 chromatogram?

More Ram Hari Dahal's questions See All

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Is it fare for editors of reputed Journals to put on hold manuscripts more than six months without review updates, Are they harassing researchers?

It is being seen that Editors of some reputed Journals are putting on hold the manuscripts and not updating review states even passing more than six months from submission. Researchers are...

24 July 2024 8,596 2 View

Can I Use D-PBS for Preparing a 10% Sucrose Solution for Lentivirus Concentration?

Hello, I am a master's student currently conducting lentivirus transduction experiments in Jurkat cells. The virus is not effectively entering the Jurkat cells, so I am planning to use an...

21 July 2024 4,676 4 View

Unification of General Relativity and Quantum Mechanics is possible ?

How can anyone develop a theoretical framework that successfully unifies general relativity and quantum mechanics? how this new theory should accurately describe gravitational interactions at...

10 July 2024 5,040 8 View

The field of cosmology and general relativity is vast and intricate,mistakes in tensor calculations, which are fundamental in general relativity ?

The field of cosmology and general relativity is vast and intricate, with numerous potential areas for misconceptions and errors. Initial mistakes in tensor calculations are some common mistakes ?

27 June 2024 6,329 2 View

On 27 May 2024, accidentally I removed 25 papers from my list of publications on Rsearch Gate. How to restore deleted papers?

28 May 2024 3,927 1 View

Sulphur test method for edible oil?

Hi everyone do you have any method for sulphur test in edible oil? as per lower cost, which instrument we can analyze sulphur in edible oil?

29 April 2024 9,789 0 View

How to write a research paper?

please tell the research paper writing steps from beginning to end.

24 April 2024 978 5 View

Explain the geological cycle of formation of different types of rocks .what are the physical ,chemical,and biological phenomenon involved in forming?

need answer in a tleast 20 lines

21 April 2024 5,489 0 View

I treated CNTs in reduced Nickel chloride solution using sodium boro hydride. After centrifuing i got mixture of CNT and nickel. how to seperate CNT?

I treated CNTs in reduced Nickel chloride solution using sodium boro hydride. After centrifuing i got mixture of CNT and nickel. how to seperate CNT? i have done this for decorating CNTs wit...

20 April 2024 6,574 1 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

AUX gas reading problem on QE with full MS and PRM method in one run?

Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...

06 August 2024 4,953 0 View

Absorption coefficient of methane?

Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?

06 August 2024 980 5 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

Why is TLC not a form of partition chromatography?

Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?

03 August 2024 7,035 3 View

Is there any Indian Company or Startups, from any universiy or IITS, which manufacture Gas Chromatography instrument , i want to make a custOmized G?

or we can collaborate

01 August 2024 6,705 2 View

Jai Ghosh

It is not all that simple to determine the menaquinones from the chromatogram pictures, unless you compare it with either purified samples or primary standards if available.

Bhanwar Singh Choudhary

If you have pure menaquinone, then first inject the known quantity of that, after that measure on the basis of area of pure menaquinone and your sample.