Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What information can be had from raman at higher wavenumber?

i have recorded raman spectra of strontium oxide thin film. the spectra were recorded in the range 400 to 4000 cm-1. i have reference to peaks upto 1000cm-1. but i couldn't find literature for...

26 February 2021 2,499 1 View

What are the methods used for CNT..?

Respected Professor's i hope you are doing well and happy. i need some information about carbon Nanotubes. before blending the CNT with the matrix the CNT has been introducing in some treatments....

24 February 2021 5,383 5 View

Langevin Dynamics in shear flow?

Hi, We are trying to simulate particles with Langevin dynamics in a shear flow by Lees-Edwards boundary conditions in MD simulations. It seems thermal forces and shear flow do not go hand in...

23 February 2021 1,761 1 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Which is the best software or programming language (user friendly) for analysing data?

I have a basic knowledge on Python, R and MATLAB in analysing data. Among those, which is the best and user friendly? Which one should I learn deeply to analyse data?N:B: I want to learn data...

20 February 2021 3,950 15 View

Hi..how to isolate crude HDL (all subfractions) from human serum by size exclusion chromatography?

I would like to know about isolation of crude HDL from serum by SEC. Is it possible to perform with a serum sample volume of

19 February 2021 5,576 2 View

What is the reason film thickness increased?

what is the reason film thickness increased when increased doping concentrations give some reasons The measured film thickness is 156, 282, 394, 548 and 681 nm for 0, 1.5, 2.5, 3.5, and 4.5 wt....

17 February 2021 8,987 5 View

Do you have any experience with transfection of EBV immortalized B cells with Lipofectamine?

I know that efficiency of transfection for primary B cells is very low with Lipofectamine. But I did not try that for EBV immortalized B cells. Do you have any experience with transfection of EBV...

05 February 2021 4,159 1 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

What are damage parameters for SMA while modelling on ABAQUS 2020?

I have given Elastic and Superelastic material properties, but I think we need to provide the damage parameters also. Please help me here.

16 February 2021 8,694 1 View

How to estimate interrater-reliability of a variable with 3 columns of answer?

Hello everyone, In my study, I asked participants to write 3 different memories. After collecting data, 2 raters coded these memories. Now I have 3 columns for the first rater and 3 columns for...

06 February 2021 7,463 8 View

What is the appropriate role of memorization in foreign language teaching?

Research in FLT has always been concerned with the role of memory in learning and the relationship between memorization and learning.

04 February 2021 2,202 5 View

Help me conduct a neuroscience experiment for my master's thesis?

22 January 2021 9,994 5 View

What are the existing NLP techniques that can be used to extract use cases from a software requirement text?

according to findings so far Micro understanding is the extracting of individual entities, facts, or relationships from the text. so findings approaches and algorithms to performing that provide...

20 January 2021 8,378 3 View

Which neuropsychological tests assess immediate and delay(24-48hrs) declarative memory/learning (verbal and visual) and procedural memory in children?

it can be either a neuropsychological assessmentfrom age 8 years old or a wel research paradigm

13 January 2021 2,308 2 View

What is the need of having weights at the output layer of an RNN ?

In RNN, Yt is the output which is given by the equation; Yt = Why.ht where Why is the weight associated with the output layer and ht is the current state. What is the need of having this weight...

11 January 2021 8,710 4 View

How to interact with internal memory of FPGA and connect it a signal or array inside a module?

I am trying to implement a processor tile on FPGA using vivado but it is taking too long to synthesize design due to large amount of Instruction and Data Memory. Currently, I have put complete...

05 January 2021 8,349 1 View

How to solve "Too many atoms in a patch" error?

Hi all, I was trying to run a simulation in NAMD but encountered the following error: FATAL ERROR: CudaTileListKernel::buildTileLists, maximum shared memory allocation exceeded. Too many atoms in...

01 January 2021 6,491 3 View