Is there a standard way of preparing stock solution of uric acid for uv spectrophotometry determination? The glycine buffer is to be prepared with NaOH, and the uricase with the buffer. Most papers prepare the uric acid stock standard with lithium carbonate. Can it prepared with NaOH instead, and if can how much the concentration?
Method by the use of NaOH is reported as follows:
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Preparation of standards
[1]Preparation of a 100 mg/L stock solution of uric acid
Weigh out 100 mg uric acid, transfer it to a 1 L volumetric flash, add about 900 ml distilled water, and then add about 100 µl of 0.6 N NaOH to help dissolve the uric acid. Make up to the volume with water when uric acid is fully dissolved
[2]Dilute the stock solution to give working concentrations of 10, 20, 30, 40, 50, 60 mg/L.
[3]If you do this analysis routinely, it is better to prepare standard solution in larger volumes (500 ml or 1 L).
[3]Store each working standards as small aliquots in the freezer.
[4]Only the necessary quantities are thawed and any left over discarded. This ensures that fresh standards are used for each analysis run.
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- Absorption Spectroscopy
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