How do I perform RNA sequencing with cancer cell lines and plant extracts?

More Patricia N Nabende's questions See All

How do I add more research done?

How can I add additional published work?

03 June 2024 4,045 2 View

Do you know of any questionnaire that can measure the glass ceiling in female engineers and another that can measure their work performance?

Greetings everyone, I am doing a research project on the effect of the glass ceiling on the work performance of women engineers in the construction sector. Do you know of any questionnaire that...

01 June 2024 7,226 0 View

Is my protocol effective for extraction of protein from cell fractions?

Fractionation 1. Scrape cells and collect by centrifugation (500 × g, 5 min, 4 °C) 2. Resuspend cell pellets in lysis buffer and incubate on ice for 10 minutes 3. Centrifuge at 500 x g for 5 min...

25 March 2024 6,347 3 View

The connection between learning and physical movement for students with disabilities?

I am writing a master's paper looking at the connection between learning and physical movement for students with disabilities. Have you run across any recent studies or information?

12 March 2024 835 0 View

What is the most efficient way to exchange inside conditions of liposomes?

I want to dry down a large quantity of liposomes and aliquot and freeze. I want to be able to play around with the inside conditions, changing buffers, dyes, etc. I can make a 2X buffer to hydrate...

11 March 2024 7,157 0 View

¿Qué técnicas de investigación podríamos utilizar para estudiar los abusos sexuales en menores?

Pretendo investigar cuál sería el método que más se adapta a la investigación, todo ello desde una perspectiva cuantitativa y cualitativa. Teniendo en cuenta los estereotipos de género y las...

11 March 2024 7,376 0 View

Use of metronidazole in the treatment of leg ulcers ?

Use of metronidazole in leg ulcers

01 March 2024 5,775 2 View

Is there a non pharmacological treatment for infected leg ulcers?

non pharmacological treatment for infected leg ulcers

01 March 2024 8,671 2 View

Does this graph look right for the Electrochemical workstation anaylsis?

I used: .01M Phenylalanine. .1 M Ascorbic acid

20 February 2024 6,790 0 View

Immobilization of E. faecalis biofilms on glass slides for AFM imaging in liquid?

I'm trying to scan the surface of E. faecalis biofilms on glass slides however when using AFM in liquid mode, I get nothing. My theory is that E. faecalis produce weak biofilms that lift off as...

20 February 2024 4,959 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

May members post flyers about opportunities to present at a conference? If so, where to post?

May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/

08 August 2024 4,585 1 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View