ANSYS, how is far-field acoustic pressure calculated (from which underlying surface)?

In ANSYS, as in also e.g. COMSOL Multiphysics, you can calculate a far-field acoustic pressure outside the explicit FEM domain. However, whereas in COMSOL Multiphysics you define a certain closed...

04 January 2021 3,076 8 View

Band size NFkB of app. 150kDa??

I made a western blot with the p65 NFkB antibody and the phospho p65 NFkB antibody from cell signalling. I get a band for both the phospho and non-phospho antibody at around 150kDa, how can this...

16 November 2020 1,948 3 View

Current clinical trials on human ehrlichiosis and the long term effects of this disease/viable treatments aside from doxycyline?

I'm working on a personal project concerning human ehrlichiosis (more common in animals). There isn't much research available that I've found on the long term effects of the disease with minimal...

23 October 2018 3,464 2 View

How to do support vector machine based feature/variable selection?

A question that has been bugging me for a long time is how to use support vector machines for identifying highly important features/variables for a given classification or regression problem? I...

04 October 2016 3,032 5 View

96-well total genomic DNA extraction protocol suggestions?

We are looking for a reliable and established total genomic DNA extraction protocol for adherent cells on a 96-well plate. Any suggestions?

02 June 2016 791 3 View

How do we detect CRISPR/Cas9-induced genomic cleavage in heterozygous cell lines?

I'm using the CRISPR/Cas9 gene editing method to target a gene in human iPSCs for mutation correction via the homology directed repair pathway. I want to compare the efficiency of different gRNAs...

10 April 2016 881 2 View

ANOVA, T-Test or Chi-Squared?

02 December 2014 8,459 7 View

How can I optimize the number of ensembles when applying a bagging procedure with any given supervised learning algorithm?

I am using bagging for machine learning classification, and would like to know how to decide what is the optimal number of ensembles. I would also like to take into account the number of positive...

20 October 2014 4,951 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View