I work on an ion chromatography system for the separation of anionic species in wine. Since I changed the Guard and Separation Colums, backpressure increased from 1500 to 1900 psi at my working flow (1 ml/min) and I got a poor resolution of closer peaks in the chromatogram. How do I understand which is the cause of this problem? The instrument manual suggests to detach every component and see if it's the responsible of the high backpressure, but I only know the backpressure value of the two columns and not that of the tubings and other components. Moreover, some components are not detachable because they are in the back of the instrument and I can't reach them.