09 September 2019 3 5K Report

I'm working on extraction RNA from S. cerevisiae and no matter what I do, my 260/280 looks like this. I'm not asking about the 260/280 itself, because I'm thinking my cells aren't lysing completely, among other things that it could be. My question is how I get rid of the phenol contamination (left shoulder)? Yes, I use chloroform every time. I've tried using columns, not using columns, extra EtOH washes, etc. I don't ever come close to disrupting the interphase when I'm removing the RNA aqueous layer. I don't understand why I can't get rid of that Trizol. Thanks in advance.

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