After turning on the AKTA FPLC, I noticed that the UPC-900 read out the following error message after the self-calibration failed: “73 WARNING Too much straylight leaks in”. The OD ranges from 1000-6000 mAU with double distilled water. My adviser and I replaced the UV lamp and tested both 254 and 280 nm filter housings along with the light housings. The flow cell tubings are non-transparent and the optical unit is not exposed to direct light (the FPLC is kept in a 4°C cold cabinet with no light source). The FPLC is an older model and is no longer serviceable. Are there any suggestions on how to fix the straylight issue?
I also forgot to mention that the UV flow cell was removed, inspected and washed, then placed back into position.
Hi, were you able to fix the problem? I am having the same error message in our UPC-900 unit, and I have not figured out how to solve it
Hello Roman Bonet, we are still trying to figure out this mystery. We are looking into changing the 254/280 nm filter at the moment.
Hi Lynnicia Massenburg , were you able to fix this issue? We just ran into it ourselves, and wanted to know whether or not you were successful. Thanks, Andor
Hello Andor J Kiss nope, not yet. We have a backup FPLC to use for now, but I'm interested to see what is going on with this straylight issue.
- Badges
- Science topic