How to use protein A G beads to purify antibody?

Secretory universal influenza antibody CR9114 were successfully expressed by adenovirus vector ,then we used protein A G beads purify CR9114.But the molecular weight of the purified antibody under...

22 February 2021 4,318 2 View

Amorphous silica XRD peak at around 10 degree?

21 February 2021 3,417 5 View

How could I get the P-value from the mean, SEM, and n of different groups in the t-test?

How could I get the P-value from the mean, SEM, and n of different groups in the t-test without the original data? For detail, I would like to know the P-value between BMP-2: 50ng/ml group and the...

06 February 2021 6,539 4 View

Tutorials for Flow boiling simulation using Fluent?

Hello, I am trying to observe pressure drop oscillations during boiling flows in Ansys Fluent. To do that I am trying to get more accustomed to modeling flow boiling in the software. So far I...

01 February 2021 6,446 2 View

What mechanism is involved when chitosan is coated on magnetic nanoparticles?

Hello! I am currently working on coating of chitosan on magnetic Fe3O4 nanoparticles. I am wondering how this coating process happened? i.e. through what mechanism that chitosan can be coated on...

13 January 2021 5,657 5 View

The impact of different poisson's ratios when analysising field vibration?

The Poisson's ratio of soil is quite difficult for us geo-engineers to obtain. I came across a problem when I tried to determine the parameters in a FEM model. For the saturated clay in...

02 January 2021 8,971 3 View

Is it possible to deposition a large area dielectric layer(mm scale) on the wet transferred graphene?

I want to deposition a dielectric layer on top of the CVD wet transferred graphene. The purpose of the dielectric layer is to uniformly cover the graphene and enable the top-gate modification of...

14 December 2020 7,693 2 View

Is the fluorescent protein must be totally photobleached in FRAP assay?

Hi I have tried to measure the mobility of a protein of interest by the FRAP assay. The protein was fused with GFP. I overexpressed the fusion protein in Hela cells. However, when the area of the...

14 December 2020 6,567 1 View

Is there some effective transfection reagents or methods for transfecting plasmids to ExpiCHO-S cells?

Dear all, We want to transfect pCDH plasmid or other plasmids to suspension ExpiCHO-S cells and express our target proteins, and we used the transfection reagents PEI. Unfortunately, there...

26 November 2020 8,323 3 View

No title

The cell I used is 293T.I want to it can grow on the biocompatible materials,like agarose or alginate gels.But the shape of the cells is not good.In the T25 flask you will find the cell like...

26 November 2020 1,009 2 View

Can I put a figure that was drawn by Bio-Render in publication paper?

I want to publish paper in journal but I have figures which have drawn with bio-renders, can I put them in the paper or which other software I can use to draw them?

27 February 2021 8,179 1 View

Recommendations for sample size in visual qualitative research (using children's drawings)?

Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...

07 February 2021 9,530 3 View

Best way to draw COLONY output / pedigrees?

Hi, I am using the sibship analysis software COLONY 2.0.6.6 (most recent version) on about 150 individuals offspring, with some of them as potential fathers and mothers. COLONY provides a lot...

02 February 2021 6,910 2 View

How to plot 3D plot for Measured value of antenna?

Hello everyone, I am trying to plot a 3D pattern from measured data of antenna in the MATLAB, for this, I have many files for one GHz frequency at different theta(e.g theta 90,80,70, etc). My...

31 January 2021 8,458 2 View

Why can't ANSYS Mechanical APDL detect the volume of my design ?

The 1st image shows the moment when I import my design(IGES) into the Mechanical APDL. The 2nd image occurs when I wanted to Operate>Boolean>Divide Volume By Area. The design is a regular...

28 January 2021 2,962 3 View

How can I plot MODIS HDF-EOS grid images into geographic coordinates (lat-lon)?

I'm analyzing Madis products (MCD64A1), when I finish my analysis, I have a 2400X2400 matrix that I want to draw, but since the original image was in sine design, when using From imagesc, draws...

14 January 2021 6,534 2 View

How can i remove the error bar from the legend?

xdata1=tempmeasS2.Timesec; ydata1=tempmeasS2.UCNP; plot (xdata1,ydata1) err2 = tempmeasS2.UCNP1; e2=errorbar(xdata1,ydata1,err2, 'b-', 'lineWidth', 1, 'HandleVisibility', 'on') legend ('1...

13 January 2021 3,140 3 View

How to Draw Crystal Structure from powder XRD?

Hi, I would appreciate if anyone can recommend on the process of drawing crystal structure from observed powder xrd diffractogram and if there is any suggested software to work on.

05 January 2021 3,517 3 View

How to construct a combined contour plot of two different text files in python?

As I constructed sperate contour plot for each methane and ethanol but now I wanna draw a combined plot for methane/ethanol ratio (for selectivity purpose)?

30 December 2020 4,531 1 View

Which software or online program is best to draw rarefaction curve?

Rarefaction curve and its uses regarding abundance analysis. I will be thankful to you.

28 December 2020 2,790 1 View