for example, the needed dose is 400ug/mL, how will I turn my crude extract into 400 ug/mL? p.s my research is about a-glucosidase activity
To convert your crude extract into a concentration of 400 ug/mL for your research on α-glucosidase activity, you will need to determine the current concentration of your extract. Once you have this information, you can calculate the volume of your crude extract needed to achieve the desired concentration. For example, if your crude extract concentration is 800 ug/mL, you would dilute it by adding an equal volume of a suitable diluent (e.g., water or buffer) to obtain a concentration of 400 ug/mL. Alternatively, if your crude extract concentration is higher or lower than 800 ug/mL, you can adjust the volume accordingly to achieve the desired concentration of 400 ug/mL. It is crucial to ensure accurate measurements and proper mixing during the dilution process to achieve reliable results for your α-glucosidase activity research.
how do I determine the current concentration of my extract? is it by computing for the percentage yield?
To compute the dose:
Identify the type of dose.
Gather relevant data.
Use the appropriate formula or model.
Apply the formula to calculate the dose.
Interpret the results based on guidelines or limits.