If the linear range for an electrochemical sensor is 700 nM - 300 M how to calculate limit of detection for the same
Please specify it in detail
I agree with above. And signal to noise ratio should be greater than 3 and less than 10.you can find in details in the following book:
Statistics and Chemometrics for Analytical Chemistry by Miller & Miller
or in a simple video: https://www.youtube.com/watch?v=8z-2oK-iJAs
Signal from lowest concentration that you can detect (detection limit) should be greater than average of electrochemical signal from zero conc. + 3* (Standard deviation of signal from zero conc.)
obtain at least 10 blank electrode response without analyte, then calculate standart deviation of 10 blank electode response, then there is an equation LOD(limit of detection) 3 times of standart deviation """"(3xSD)/m"""" m is the slope of calibration curve. the calculated result is the limit of detection
This paper define the detection limit (LOD), you can calculate the detection limit for an electrochemical sensor using their method, (Thévenot D R, Toth K, Durst R A, et al. Electrochemical biosensors: recommended definitions and classification[J]. Biosensors and Bioelectronics, 2001, 16(1): 121-131).
See this paper for more LOD definitions depending on how your data have been acquired: alternatives to acquire ten folds the blank and more...
http://dx.doi.org/10.1016/j.snb.2012.06.071
Sensitivity is a key performance parameter of biosensors, Sensitivity is defined as the slope of change in signal(current) with a change in concentration:
- S = (d )signal/d(concentration).
Record series of blank responses without adding analyte and then calculate the St. Dev. for these electrodes. Take the standard deviation and multiply it by 3 and then dived by slope.
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