I want to rescue some old Arabidopsis seeds. I have tried several days vernalization and treatment with GA3, but there is no germination. Would you have some other ideas of what could I do? Thank you.
Hi Laura,
Take a look at the suggestions from this question on RG: "I want to germinate 10 years old Arabidopsis seed, anyone can suggest me about this?"
https://www.researchgate.net/post/I_want_to_germinate_10_years_old_Arabidopsis_seed_Any_One_can_suggest_me_about_This
Hi Laura,
Take a look at the suggestions from this question on RG: "I want to germinate 10 years old Arabidopsis seed, anyone can suggest me about this?"
https://www.researchgate.net/post/I_want_to_germinate_10_years_old_Arabidopsis_seed_Any_One_can_suggest_me_about_This
Dissolve 20 mg of gibberellic acid in 60 ml of water (concentration 300 ppm), and soak the old seeds.
tetrazolium test to determine the viability,May be due to the aging of Seed Analysis of phenolic compounds, anthocyanins, ascorbic acid, so in my opinion the use of tissue culture containing sucrose as well as the viability of alpha-amylase can be effective.
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