we have a microbiology reader (LOGPHASE 600, BioTek), but unfortunately, we can not draw a growth curve for SC and SCEG BUT it works for YPD and YPEG. If you had a similar experience, kindly let me know and provide your solution in writing.
The question does not have enough details. Is the problem that the strain does not grow at all in SC and SCEG? Or that the data is not reproducible, or the curves don't look correct? Those are all very different sorts of problems.