I am no expert in industrial on-line monitoring. In laboratory conditions in principle you can use both IR microscopy or Raman microscopy. Confocal Raman Microscopy or NIR microscopy may also be useful for your purposes. Plenty of literature  can be found by Google.

Dear Chang, to determine of reactive groups you can employ FTIR- ATR- XPS.

Dear Sir,

FTIR technique is better for the detection of functional groups.

Dear All, thanks for your kindness reply. Traditional IR or or Raman method are popular for functional groups study, but I am looking for Fast on-line method and space distributions of functional groups for industry application. We don't need to have precisely data, just roughly analysis is enough.

Then I would suggest NIR camera with multiple wawelength detectors. You will have to establish, however, a correlation between the functional groups and the specific absorptions at various wavelength. (Chemometry, calibration)

https://www.whitebearphotonics.com/multispectral-cameras-1

http://onlinelibrary.wiley.com/doi/10.1002/ange.201200840/abstract

https://macsphere.mcmaster.ca/bitstream/11375/6173/1/fulltext.pdf (this is esepecially relevant)

Dear Dr. Banhegyi

We deeply appreicated your kindness suggestions and help. I think that might be help for our purpose. I can't wait to study and try it.

I would also suggest confocal RAMAN microscopy, however, there can be issues with signal strength if the polymer fluoresces (this also depends on the wavelength of the laser). It is also possible using this technique to map a surface area but this takes a long time.  

It depends on the thickness and the quality of answer you require.

for a thickness of 1 to 10nm you can apply :

- Tof-SIMS measurements (< 1nm depth and  1 micron lateral resolution) with semi quantitative measurement of chemical species

- XPS on < 10 nm depth for chemical composition and deconvolution to extract the chical functions

For a thickness ~2 microns Infrared imager (not infrared microscope) in ATR mode is able to deliver a chemical map of the chemical species. This method is efficient also for surface treatment analysis. The lateral resolution is about 3 microns (it dependson the apparatus)

Dear all,

I think Atomic Force Microscopy (AFM) may also be usefull. Regards

If you want only a fast and cualitative method; y You can put a Little piece of film in toluidine blue solution and see it visualy, or with an optical microscopy, the blue color surface. If you put one solution with exact concentration of toluidine, then you can determine it by volumetric determination.

The best method is by XPS, but it is not to fast.

Mr C.C is asking specifically about on-line, fast measurement tool that can do mapping of functional group on a substrate. So, I am presuming he is looking to probe first few micron thickness of a substrate that conatins various functional groups. XPS/ESCA and TOF-SIMS are the two best analytical tool available to do such study but unfortunately both of these techniques are not fast and can not be used on-line. I am not aware of any commercial tool that can meet your requirements.

Now, ATR-FTIR can be used for such purpose but you will have to build a moving stage if you want to do mapping of the entire surface (this can be done) but again its not a 5 sec measurement tool. In recent years, a lot of publication have appeared that seem to indicate that Chemical Force Microscopy can be used for such study. CFM is basically a high resolution AFM with chemically modified tip that can be used for mapping of functional groups on a substrate. Please see following ref.: A. Noy et. al, Annu. Rev. Mater. Sci., 27, pg 381 (1997); Schonherr et. al., Macromolecules, 33(12), pg 4532 (2000); Schonherr et. al., Chemical Comm., 14 (2000); Advances in polymer Scciens-Vol 182, Polymer analysis/Polyemr theory, ed. Akihiro Abe and D. Shiro, Springer (2005), pg. 73 ff. These authors mapped the spatial arrangement of different functional end groups exposed on a polymer surface (mono layer). I am not sure if this can be automated to do what you want, but possibly may be useful to consider this tool. Talk to folks at Park Systems and Nanocraft who sell these equipment. They may be able to make automated stage that can probe bigger surface area that may meet your needs. 

Dear Dr. Prasad

Thanks for pointing new possibility for our requirement. we will check if CFM is good candidate.

we want to find out if there is an appropriate tool which can on-line provide 2D image of distributions of functional end groups on the surface of polymer or plastic plate. the image size can cover 300 x 300 mm real surface is good enough, the 2D information doesn't need high space resolution, just roughly is enough, furthermore,  just can show difference of concentration of functional groups for one kind of functional groups between articles or products is enough for us.

So, I think CFM is worth a try. Based on your chemistry the manufacturer can build a AFM tip that will work for your system.  talk to representatives from park Systems. 

Dear Dr. Chia

CFM may work, see following links

http://www.annualreviews.org/doi/abs/10.1146/annurev.matsci.27.1.381

http://pubs.acs.org/doi/abs/10.1021/ja963375m

Dear Dr. Sharma

thanks for your kindness information about  CFM

May I ask if CFM can on-line quick provide 2D image information? Analyzing size is about 300 x 300 mm