The aeromonas hydrophila colonies were isolated from the hemorrhages as well as the body fluids of the infected fish. Triplicate samples were aseptically blended with Tryptic Soy Broth (TSB) (Hi media,India) and incubated at 370C overnight for enrichment. The culture was plated on Tryptic SoyAgar (TSA). The other colonies also identified byusing nutrient broth and the culture were grown innutrient agar. The selected isolates were identified by morphological, physiological and biochemical confirmations (Farmer and Hickman-Brenner,1992) as well as based on the characteristicsdescribed in Bergey’s Manual of SystematicBacteriology (Holt

et al., 1994). Further it was stored in nutrient agar slants at 40 C for further experiments.

Thank you so much sir