If you develop immunity and it will be response is more but only short periods how long it will be maintain? should be ????

Immunize intraperitoneally with an antigen, wait six months, and then test memory B cells from head kidney leukocytes in vitro for the production of IgM by ELISPOT

why??????? with in the two week we will expect IgM production.......may be iam right.......

Waiting until six months to be sure that the Ab titre went down and hen you can test the cell's memory for the same Ag you used in the first time.