hello everyone, I was wondering if anyone knows any method or protocol to synchronize CD8 T cells activated with peptide, because I want to mark them with cell trace violet and grow them in vitro to evaluate peaks of proliferation.
Are there mycologists here (or researchers who carry out research involving fungi) based at the UNIVERSITY OF SEVILLA or the UNIVERSITY OF CADIZ?
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i need a Scientific Paper Research topic with hypothesis and statistical computation
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Networking Learning community Social interactions for cooperative learning
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I am interested in relevant archaeological evidences from any areas and periods.
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I want to inject fly embryos with a collection of 50 different RNAs that I would like to in vitro transcribe from the lowest number of plasmids. If I insert in the plasmid 5 sequences (or more if...
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I wanted to expand purchased iPSC. According to my protocol I should not use antibiotics. I am expanding them under feeder free conditions (coating with Geltrex) and using complete Essential 8...
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I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
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Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
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During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
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After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
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I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
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I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
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Dear All, My lab primarily works on insect wing patterns. In one of the projects, my student and I have defined 19 abnormality characters on the forewing and 6 abnormality characters on the...
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Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
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I have two groups that I test on three different tasks. I have 4 independent t-test, 6 paired t-test and 2 ANCOVA. My concern for which t-test should I conduct bonferroni correction. At the moment...
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Hey researchers! I am currently doing a research about to what extent in which A has accelerated the inclusiveness of the payment system in my country. Down below are a few examples of the...
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