hello, I am master degree researcher from South Korea.
I made some materials following another researchers.
But unfortunately, my reaction needs DMSO for solvent.
as you know, DMSO has very high BP(around 180oC, as I know of).
I used vaccum rotary evap to evaporate DMSO but I failed.
also I used vaccum oven around 120oC, it has failed too.
finally I precipitated mixture with poor solvent, but it still had some traces of DMSO.
The reason why I've been trying to get rid of DMSO out of my sample is to get solid state of it.
The trace make it very sticky liquid state so it's hard to applicate anywhere else.
My sample is very soluble in water, so I doubt water-wash can extract DMSO from my sample.
How can this get done?? Please give me some advices.
and sorry for my poor english skills :(
Is your sample soluble in alcohols? You can try to remove DMSO by alcohol. I conjugated FITC to dextran-coated nanoparticles in DMSO, then carefully washed out the by-products and precursors with anhydrous i-propanol, since the carbohydrates are insoluble in alcohol (although soluble in water).
Good Luck!
I would think 'dialysis'. Maybe it has already been performed. Do an internet search,
Do I understand correctly, that you want to get rid of DMSO, w/o heating the reaction solution to 180 °C?
In case your products are not volatile, you might want to try vacuum distillation, aka "speedvac". The sample is cooled during the automated evacuation process...
Hope this is what you have in mind?
Cheers,
Detlef.
As suggested by Dr. Jensen, speed vac is the way to go. You can freeze your sample and then dry it undet vacuum. We always do it in our lab to remove dmso from samples.
Dear Dr.Jensen and Navid! I appreciate your answers but our lab cannot operate freeze drying or speedvac.
what we have for evaporating or removing solvent is just rotary evap(but the bath temp cannot go over 100oC) and vacuum oven.
do you think heating up to 180oC in vacuum oven is not dangerous?
or how about using vacuum oven after diethyl ether - water extraction with sep. funnel several times?
I really appreciate of all of your precious time to answer me..
...the reality of life kicks in... As you are at a University, why not look if other departments or faculties might have access to this approach?
To my understanding vacuum ovens are NOT designed to remove organic solvents. Don't use it until you have checked its application with the vendor of the device. Do NOT use any technology that could potentially lead to explosive vapors this is far too dangerous.
Always use certified and proven technology fit for the respective purpose. Check with your professor about the availability of professional vacuum distills or speedvacs in the vicinity of your laboratory. Play safe!
Dissolve your product in dichloromethane and add water (shake it well), then you will fine two layers, remove water layer, repeat this water workup several time, you will find your product free of DMSO......if you need more help, let me know
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