How can I purify the protein fraction from a plant sample containing mostly polysaccharides?

06 June 2015 1 308 Report

Trying to do some proteomics from a very viscous plant sample containing mostly a mixture of high molecular weight polysaccharides. Traditional methods to precipitate the proteins like TCA/acetone or salting out result in co-precipitation of some of the polysaccharides too. I thought on SEC but it is tedious... maybe some kind of filtration around 300 Kda? Any other alternative?

Oluwatobi T. Somade

You can boil the defatted sample with 80% alcohol solution, to remove monosaccharides and oligosaccharides. You will be left with proteins and polysaccharides, you can then precipitate out your protein using various techniques like salting out, isoelectric precipitation, solvent extraction......

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