To make easier the interpretation of the age in the otoliths of horse mackerels, these are immersed in a mixture of glycerin and alcohol. After that, otoliths become dehydrated and I would like to know how I can keep them for further readings.
You can find some useful information in www.marinebiodiversity.ca. (STEVENSON, D.K. and S.E. CAMPANA [ed]. 1992. Otolith microstructure examination and analysis. Can. Spec. Publ. Fish. Aquat. Sci. 117: 130 pp)
Not too long to prevent it being brittle or disintegrate.
Keep in dry, in vials ir envelop,
immerse in resin before examination.
Sorry: Keep it dry, in vials or envelop. If time permits, immerse in resin.
Thank you both, Dr Amini and Dr Ambak. These documents will be useful.
I was informed by my senior collegue Dr. Mohsen Hussaini in KISR who has vast experience, U should keep them in dessiccation bags (keep away from moisture) is the best way.
For large otoliths like is common with marine fishes, dry preservation is recommended; but for smaller and delicate otoliths as found in fresh water fishes, preservation in ethanol is recommended.
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