How can I modify or create frame work for IOT Applications ?

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Do you mix and add all primers together in a singel PCR reaction (4 total primers for 2 mutations) when using Agilent's multi-site mutagenesis kit?

I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...

25 July 2024 6,517 3 View

Why farmer's of India not adopting Integrated farming systems?

While IFS is beneficial

15 May 2024 4,174 4 View

What is the approach for Machine learning algorithms selection for material science?

Which Machine learning algorithms suits best in the material science for the problems that aims to determine the properties and functions of existing materials. Eg. typical problem of...

10 May 2024 1,490 3 View

How do we contruct an optimal feature set?

How do we evaluate the importance of individual features for a specific property using ML algorithms (say using GBR) and construct an optimal features set for our problem. image taken from:...

10 May 2024 2,691 4 View

To analyze intestine, is IEC isolation the only way?

I'm currently analyzing the intestine using villin CRE knockout mice. While my team specializes in liver research and we're expanding into intestinal studies, we're not very familiar with...

18 April 2024 3,776 1 View

What will be the effect of using the low thermal conducitvity PCMs at fixed thermal conductivity of 1 W/(m K)?

The wide application of PCMs is rather limited due to a very low conductivity (less than 1 W/(m K)). If we fix the thermal conductivity of all these PCMs at 1 W/(m K). Then what will be the effect...

07 February 2024 3,593 1 View

I have an EPDM rubber that has swelled in oil. What solvents can bring back the rubber to the original shape?

24 January 2024 7,796 1 View

What is the impact of a Journal changing publishers for an author of a research article?

Dear all, I submitted a research manuscript to the "Journal of the Geological Society of India" on May 25, 2023. The paper underwent two rounds of "Major Revisions," which were successfully...

08 January 2024 2,822 0 View

Countess II FL Uneven Lighting. Any Fixes?

The lab that I work at uses a Countess II FL cell counter and the machine typically works fairly well. Recently we have noticed that the lighting for the brightfield is uneven and causes cells to...

28 September 2023 6,872 0 View

I want to deposit 5 micrometers linewidth of metal on my substate using shadow mask in sputtering technique. please suggest is it possible?

Is it possible? if any manufacturer is there please suggest. Also, suggest any other method for low work function metal deposition in 5-micron linewidth (5 microns * 5 mm).

27 September 2023 3,497 3 View

All math can be explained by iterator of code?

all math can be traversed by code? all math can be translate to code?

26 July 2024 9,530 0 View

Looking for articles that provide guidance on doing Thematic Analysis (Braun & Clarke) as a team?

Does anyone know of any published articles that provide guidance on using TA with multiple team members? I am aware of one (see below), however, there are some disagreements in terms of some of...

23 July 2024 7,819 5 View

Could anyone please guide me on to proceed to apply for phd ?

Hi Everyone , I finished my Master's in Computer Science . I have work experince in IT services and learning cloud tech . I am not a good programmer (Can do if needed) . I like scripting...

10 July 2024 267 0 View

Should HIV testing also be offered to the low prevalence populations?

With many Northern Hemisphere countries approaching or exceeding the 95-95-95 criteria and with improved accessibility of community testing centers, I wonder whether we could significantly...

09 July 2024 2,181 1 View

Which book and outline do you recommend for computational physics course for BS level ?

students already took 1. numerical methods 2. programming language 3. Probability and statistics

09 July 2024 6,271 3 View

Is it okay to use reviewed proteins?

I'm working on the proteome (from Uniprot), as you're probably aware that there are reviewed and unreviewed proteins, however unreviewed proteins are in large quantities, but reviewed proteins are...

09 July 2024 5,782 2 View

Why does our stiff biochemical ODE model in R produce unreasonable results (negative values, NAM) despite using solvers like lsoda, vode, and rk4)?

We have developed an ODE model comprising 25 interrelated equations with common coefficients. This biochemical model, applied in wastewater treatment, is characterized by stiffness. Utilizing the...

06 July 2024 7,077 4 View

Which is better for the student : Implementing the principles of object-oriented programming using Java or C++?

Object-Oriented Programming

29 June 2024 4,877 12 View

How to design an online training, learning platform ?

when designing an e-learning platform what model and programming language do you select?

29 June 2024 7,504 4 View

How to reconstruct original observations using PCA?

I ran PCA on 4 variables using the prcomp library. All variables were normalized to have a mean of zero and a standard deviation of one (z-score) before the PCA. prc 1 and I performed a varimax...

26 June 2024 6,792 1 View

David Alexander Urrego-Higuita

Hello Ankur Bang , the best languages for IoT applications are C, java and Python(1), and frameworks Elipse Kura, Node-RED and Flogo (2).

1. https://www.iotforall.com/2018-top-3-programming-languages-iot-development/

2. https://dzone.com/articles/3-best-iot-frameworks-for-beginners

Uzair Aalam

Arduino (a micro controller based development board) is best to begin with. Learn C++ to program Arduino and then use python to interface the Arduino.

Arduino enables a program to talk to physical devices like relays etc. and python is easier for interfacing USB port where Arduino is usually connected.

Mahamadou Kante

I would highly recommend Arduino like Uzair Aalam.

Bhagyshree Pawde

A comprehensive framework can be got using a combination of MATLAB, NetSim and IBM Node red/IBM Watson Quick start.

Abdelkader Mohamed Elsayed

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