How can i model column with load at infinity distance?

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Hi, please what is the best tool on Aspen plus that can help me get the best inlet temperature and pressure for cryogenic distillation?

I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...

06 August 2024 4,611 0 View

How to perform EEG source analysis on each trial of data separately?

Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...

30 July 2024 2,744 1 View

A positively charged and 10x his-tagged protein that doesn't bind to any chromatographic resin?

Hello everyone, I am currently working on a protein that is 10x his-tagged and positively charged (predicted pI=10.16). But when I tried to use Ni-NTA column to purify the protein, it's not...

24 July 2024 7,293 4 View

Why is trifluoroacetic acid (TFA) used in c-18 column?

I would like to know the role of the TFA (trifluoroacetic acid) used on reverse phase chromatography c-18 column. It's is often used with a concentration of 0.1% on the mobile phase.

23 July 2024 8,075 3 View

Standard population: What standard population to implement?

Dear all, I have a question about standard population? Is Segi population outdated? I have a time series from 2000 onward. Regarding who 2000-2025 population from this link...

13 July 2024 4,380 2 View

How can I regenerate a SCX column modified with Sulfonate group (Thermo 054995)? The colmun has lost its Resolution and peak to valley ratio?

The column is used in Ion Exchange Chromatography of Monoclonal antibodies and the method of analysis is based on pH gradint.

13 July 2024 7,462 1 View

Jumaa Salman Chiad

https://www.google.com/search?q=model+column+with+load+at+infinity+distance?+%22pdf%22&tbm=isch&tbo=u&source=univ&sa=X&ved=0ahUKEwiSmbqwzJjbAhUmJpoKHZLqBvgQsAQIJQ&biw=1525&bih=730It is a very interested question. These links may help you .

http://nptel.ac.in/courses/105105104/pdf/m10l23.pdf

https://pdfs.semanticscholar.org/a71e/69bb394fddb6e2bcf728cbdd35b6342c062c.pdf

http://publications.lib.chalmers.se/records/fulltext/176734/local_176734.pdf

Pedro Martinez-Vazquez

A load applied at infinite distance would be equivalent to say that the column is subject to pure bending.

Mohannad H. Al-Sherrawi

Model you column as a beam, this may be helpful.