How can I select the best plasmid after sequencing?

I have transformed a drought-related gene of 1.7kb into pGEMt vector (E.coli DH5 alpha strain). After sequencing the plasmids (3 replicates) 4 changed amino acids are visible at different...

06 February 2021 336 3 View

What can be the problem in the isolation of Plasmid?

I've tried transformation of a 1.7 kb gene with DH5 alpha strain of competent cells and also used PuC19 as a control. After selecting colonies and broth-culturing overnight, I isolated plasmid....

27 December 2020 3,596 5 View

MiRNA seq differential analysis?

Hello, I am writing to ask your help in regard to performing differential analysis to analyze miR RNA-seq data. I have miRNA seq data and I have analyzed in the following way- Initial QC - removed...

19 August 2020 5,105 7 View

How to calculate RECEIVER OPERATING CHARACTERISTICS(ROC) curves and area under the curve (AUC) of microRNA data?

Hello, I am having micro RNA data sets of control and patients now I am interested into analyzing micro RNA as a biomarker for a particular disease. I have already delta delta CT values for...

30 June 2020 7,858 2 View

I can't run Mendeley desktop on Microsoft Word 2019 MSO. Does anyone have an idea?

I have added plug-in but it does not work. it shows error 429

04 May 2020 7,810 2 View

How to do web scraping of VirusTotal so that I can get the information of multiple APKs?

VirusTotal website is a tool for detecting malware Android apps. But it also gives various information related to the APK uploaded, like manifest tags, permissions etc. I want to extract these...

07 April 2020 7,228 2 View

How can i reduce primer dimers and get my cDNA PCR amplification done?

24 March 2020 1,525 3 View

Based on the figure deduce the total no. of polypeptide chains in the protein?

A purified oligomeric protein was analysed by SDS - Page under reducing and non reducing conditions. A 1 litre solution of 1mg/ml concentration has 4.01*10^18 molecules of the oligomeric protein....

15 November 2019 5,362 3 View

What is the value of M/Z at base peak of benzoic acid/?

The mass spectrum of benzoic acid will generate the fragment as a base peak (100% relative abundance) of m/z (mass to charge ratio) at?

08 November 2019 1,175 3 View

How to find molecular weight of a protein through mass spectrometry?

An uncharged protein (P) has an Asp in position 23 with a molecular weight of 6501 Da, as determined by mass spectrometry. The uncharged mutant of this protein (P') contains a single amino acid...

06 November 2019 9,961 3 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View