Thanks alot for your help.

Could you tell us what is the explant you tend to use?

Dr Robin

You try to make

in vitro culture of rosa foetida?

Dr Robin,

Yes, it is helpfull feelings from you.

I have some exeperinces about plant tissue culture.

I know that type of explant is very important to set up your experment on tissue culure

as you know,

you can have callus from any part of plant

so,

in my study, according to the target of my study, i will choose the suitable explant.

but

in most casses

we use hypocotyl or cotyledons i available, it is thought to be more easy

Thank you Dr Robin, and sorry for contruption

first of all u hv to stdy its habitats and habits,then carry on

First collect the reference regarding the rosa foetida, and what early work has been done, according to that plant your project..

https://www.google.com.my/url?sa=t&rct=j&q=&esrc=s&source=web&cd=3&ved=0CD8QFjAC&url=http%3A%2F%2Fwww.aensiweb.com%2Faeb%2F2011%2F3203-3206.pdf&ei=UFgSUcesOcjZrQeAwYDgBw&usg=AFQjCNG_B5cADuiCz6-pDbqYtzJ0Veq5mg&bvm=bv.41934586,d.bmk

Dear Reza,

I found this paper but I think this is not exactly answer.I sent mail for this person but she didnt answer.I tested many experiments on R.foetida with different explants and different Hormone concentration but it doesnt reply.

Thanks alot

Have u ever tried the other type of media?

u could also find another species close to ur plant and apply exact media but with different concentration of PGR's.