How can I exchange three nucleotides from one plasmid to other six diferent nucleotides?

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Praveen Kumar Vemuri Popular answer

You can use two different restriction enzymes to cut plasmid and re-ligate

Praveen Kumar Vemuri

Bhupendra singh Punwar

hi,

you can simply use site directed mutagenesis approach as discribed by me in attachted link

for futher detail follow link: http://sevierlab.vet.cornell.edu/resources/Stratagene-QuikchangeManual.pdf